Eosinophils promote proliferation of pulmonary structural cells by stimulating extracellular matrix production in asthma

Introduction. Eosinophilic asthma can be characterized by intense infiltration of eosinophils into the airways, where they abundantly release various cytokines, chemokines and growth factors. Airway remodeling is closely related to increased airway smooth muscle (ASM) mass; however, another important feature could changes in extracellular matrix (ECM) homeostasis, driven production ECM proteins metalloproteinases lung structural cells. Aim. To evaluate compare eosinophil adhesion ASM cells pulmonary fibroblasts or their secreted effect on cell autocrine proliferation adjustment asthma. Methods. A total 12 allergic (AA), 8 severe eosinophilic (SEA) patients 11 healthy subjects (HS) were examined. Blood isolated using high density Ficoll centrifugation magnetic separation. For each study individual combined cultures (co-cultures) between prepared. purification was performed ammonium hydroxide (NH4OH) based lysis. Eosinophils evaluated measuring peroxidase activity co-cultures; measured AlamarBlue assay. Results. In all investigated groups significantly compared (p < 0.01). Eosinophil enhanced HS group 0.05). Isolated fibroblast’ produced after co-cultures with AA, SEA promoted newly seeded fibroblast 0.01) stronger both groups. ECM, blood serum patient also AA had a than eosinophils. Conclusions. demonstrate components fibroblasts, further increases Pulmonary promote manner via released incubation